Plasmid_Backbone
Gal4::EXSP
Part:BBa_K3271003:Design
Designed by: Setti Belhouari Group: iGEM19_uOttawa (2019-10-12)
Gal4::EXSP pSB1K3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 2290
Illegal suffix found at 2312 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2290
Illegal SpeI site found at 2313
Illegal PstI site found at 2327
Illegal NotI site found at 2296
Illegal NotI site found at 2320 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2290
Illegal XhoI site found at 1026
Illegal XhoI site found at 2052 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2290
Illegal suffix found at 2313 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2290
Plasmid lacks a suffix.
Illegal XbaI site found at 2305
Illegal SpeI site found at 2313
Illegal PstI site found at 2327 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
Please note the upstream and downstream Gal4 locus homologies are found outside the prefix and suffix, not within the multiple cloning site. This contribution consists of an updated pSB1K3 BioBrick backbone that makes the BioBrick plasmids compatible with cloning in both E. coli and S. cerevisiae.
Source
The Gal4 upstream and downstream homologies are comprised of 100 bp each from the promoter region and the terminator region of the Gal4 locus of S. cerevisiae, respectively.